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If working with Gentle Cell Dissociation Reagent (GCDR), the period of GCDR treatment method will have to be optimized. Incubate organoids with GCDR for eight -10 minutes at 37°C and utilize a P1000 pipette to gently pipette up and down, and visually inspect if organoids crack up.

Mucus could be washed off the surface of your cells at the time weekly starting off at week 3, after the cells have been cultured in PneumaCult™-ALI Medium.

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The readings can also be conducted before the society is evaluated for endpoint characterizations, like electrophysiology or airway marker expression.

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Notes: STEMdiff™ Kidney Organoid Package requires a different plate structure/culture established-up 지방이식 and is not a normal monolayer culture; kidney organoids protrude with the underlying monolayer lifestyle.

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Execute cell counts applying an automated cell counter. Observe: Minimal viability is usually optimized through the size 줄기세포 지방이식 of collagenase or ACCUTASE™ incubation. It may also be optimized by reducing the amount of triturations.

Notice: PFA fixation may be considered for evaluating intracellular markers or undertaking Assessment in a later time.

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